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Friday, May 15, 2020 | History

2 edition of Improved analysis of DNA short tandem repeats with time-of-flight mass spectrometry found in the catalog.

Improved analysis of DNA short tandem repeats with time-of-flight mass spectrometry

John M. Butler

Improved analysis of DNA short tandem repeats with time-of-flight mass spectrometry

by John M. Butler

  • 292 Want to read
  • 18 Currently reading

Published by U.S. Dept. of Justice, Office of Justice Programs, National Institute of Justice in Washington, D.C .
Written in English

    Subjects:
  • Forensic genetics -- Methodology.,
  • Genetic markers.,
  • Time-of-flight mass spectrometry.,
  • DNA -- Analysis.

  • Edition Notes

    StatementJohn M. Butler and Christopher H. Becker.
    ContributionsBecker, Christopher H.
    Classifications
    LC ClassificationsRA1057.5 .B88 2001
    The Physical Object
    Paginationix, 75 p. :
    Number of Pages75
    ID Numbers
    Open LibraryOL23023833M
    LC Control Number2001344719

    and proteins, but also DNA and RNA. Tandem mass spectrometry used for sequence analysis has been can be obtained, i.e. –,56–62 and mass accuracies for DNA analysis of 30 ppm have been reported,60 It short tandem repeats (STRs) that vary by less than 1 Da.   Abstract. Currently, the use of time of flight (TOF)-mass spectrometry (MS) in quantitative analysis of small molecules is rare. Recently, the quantitative performance of TOF mass analyzers has improved due to the advancements in TOF by:

    Abstract. DNA analysis by mass spectrometry has the advantage of being the most accurate method for genotyping. Since the mass of molecular ions is measured and each allele has its own intrinsic mass, this method provides direct readout of Cited by: Time-of-flight mass spectrometers In a time-of-flight (TOF) mass spectrometer ions are formed in an ion source and accelerated by the potential difference (V) across the source into a drift region. [See Fig. 1.] Typically, a voltage of 20 kV is placed on the sample plate at the back of the source, and the ions are formed by a laser pulse whose.

    Abstract. Microsatellite genotyping has been widely used in the scientific community and has been the method of choice for many recent linkage analysis studies, especially genome scans designed to identify genes underlying common diseases (Ghosh et al. ; Risch et al. ; Xu et al. ).Microsatellite markers are simple sequence repeats consisting of repetitions of very . Mass Spectrometry is an experimental technique used to identify the components of a heterogeneous collection of biomolecules, by sensitive discrimination of their molecular masses. In matrix-assisted laser desorption / ionisation time-of-flight mass spectrometry (MALDI-TOF), the sample to be analyzed is placed in a UV-absorbing matrix pad and exposed to a short laser .


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Improved analysis of DNA short tandem repeats with time-of-flight mass spectrometry by John M. Butler Download PDF EPUB FB2

IMPROVED ANALYSIS OF DNA SHORT TANDEM REPEATS WITH TIME-OF-FLIGHT MASS SPECTROMETRY John M. Butler and Christopher H. Becker Science and Technology Research Report October NCJ Cited by: DNA Short Tandem Repeats Improved Analysis of With Time-of-Flight Mass Spectrometry science and technology research report.

U.S. Department of Justice Office of Justice Programs Seventh Street N.W. Washington, DC John Ashcroft Attorney General. Improved analysis of DNA short tandem repeats with time-of-flight mass spectrometry. Washington, DC: U.S.

Dept. of Justice, Office of Justice Programs, National Institute of Justice, [] (OCoLC) Material Type: Government publication, National government publication, Internet resource: Document Type: Book, Internet Resource.

Improved Analysis of DNA Short Tandem Repeats With Time-of-Flight Mass Spectrometry [Butler, John M., Becker, Christopher H., Justice, U.S. Department of, Programs, Office of Justice, Justice, National Institute of] on *FREE* shipping on qualifying offers.

Improved Analysis of DNA Short Tandem Repeats With Time-of-Flight Mass SpectrometryCited by: Short Oligonucleotide Mass Analysis 76 2. Method Outline 76 2. Design of PCR Primers and Fragments for Analysis 78 2.

Typical PCR Reaction Conditions 79 3. Electrospray Ionisation Mass Spectrometry 79 Formation of Ions 3. 79 3. Tandem Mass Spectrometry 79 3.

Typical ESI-MS Settings for SOMA 80 4. Purification Procedures 80 4. DNA ANALYSIS BY MALDI MASS SPECTROMETRY. (short tandem repeats (STR) Clearly mass spectrometers have improved by orders of magnitude in terms of speed and accuracy in the past ten years.

One feature that is quite striking about the use of a mass spectrometer is its use as a DNA diagnostic tool. A result essentially is the presence or Cited by: The Paperback of the Improved Analysis of DNA Short Tandem Repeats: With Time-of-Flight Mass Spectroscopy by John M.

Butler, Christopher H. Becker | at B&N Outlet Membership Educators Gift Cards Stores & Events Help. Ross P.L. and Belgrader,P. () Analysis of short tandem repeat polymorphisms in human DNA by matrix-assisted laser desorption/ionization mass spectrometry. Anal. Chem., 69, –Cited by: Like Peter Dawson's Quadrupole Mass Spectrometry and Its Applications (AIP, Woodbury, NY, and Elsevier, Amsterdam, ) and Ray March and Richard Hughes' Quadrupole Storage Mass Spectrometry (Wiley-Interscience, New York, ), Bob Cotter's chapter, page Time-of-Flight Mass Spectrometry is destined to become one of the classic Cited by: Time-of-flight mass spectrometry (TOF-MS) combined with matrix-assisted laser desorption/ionization (MALDI) can be used to rapidly genotype short tandem repeat (STR) DNA markers.

This article reviews various applications and conventional approaches for analysis of STRs and contrasts electrophoretic DNA separation methods with mass spectrometric. and Becker, C.H. () High-throughput analysis of forensic STR and SNP markers using time-of-flight mass spectrometry.

Proceedings of the Ninth International Symposium on Human IdentificationPromega Corporation, pp We use cookies to offer you a better experience, personalize content, tailor advertising, provide social media features, and better understand the use of our services. In-depth Analysis of Tandem Mass Spectrometry Data from Disparate Instrument Types * S Hence this is covering a very large search space, and the restriction to searching only a short list of proteins is necessary if large numbers (thousands) of spectral peak lists are to be searched.

time-of-flight mass spectrometer: II. Cited by:   See Butler, John M., and Christopher H. Becker, "Improved Analysis of DNA Short Tandem Repeats With Time-of-Flight Mass Spectrometry" (pdf, 82 pages), Final report to the National Institute of Justice, grant number LB-VX, OctoberNCJ   Time-of-flight mass spectrometry (TOF-MS) combined with matrix-assisted laser desorption/ionization (MALDI) can be used to rapidly genotype short tandem repeat (STR) DNA markers.

This article reviews various applications and conventional approaches for analysis of STRs and contrasts electrophoretic DNA separation methods with mass spectrometric.

improved analysis of dna short tandem repeats with time-of-flight mass spectrometry by Mass Spectrometry, John Ashcroft, John M. Butler, Christopher H. Becker, Ncj Sarah, V. Hart, Lois Tully. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been explored widely for DNA sequencing.

Compared to gel electrophoresis based sequencing systems, mass spectrometry produces very high resolution of sequencing fragments, rapid separation on microsecond time scales, and completely eliminates compressions associated Cited by: Time-of-flight mass spectrometry (TOFMS) is a method of mass spectrometry in which an ion's mass-to-charge ratio is determined via a time of flight measurement.

Ions are accelerated by an electric field of known strength. This acceleration results in an ion having the same kinetic energy as any other ion that has the same charge. The velocity of the ion depends on the mass-to. High-Throughput STR Analysis by Time-of-Flight Mass Spectrometry salts in the PCR products prior to their being introduced to the mass spectrometer (3).

Without the reduction of the salts, resolution is diminished by the presence of adducts. Salt molecules bind to the DNA during the MALDI ionization process and give rise to peaks that. Time-of-flight mass spectrometry (TOF MS) was developed in the late ’s, but until the ’s fifth and last vacuum stage, where the time-of-flight mass analysis takes place.

Because the mass short, but significant. Because the true flight. High-throughput DNA analysis by time-of-flight mass spectrometry Article (PDF Available) in Nature Medicine 3(3) April with 99 Reads How we measure 'reads'.Time-of-flight mass spectrometry (TOF MS) was developed in the late ’s, but until the ’s its popularity was limited.

Recent improvements in TOF technology, including orthogonal acceleration, ion mirrors (reflectron), and high-speed electron-ics, have significantly improved TOF resolution.

This improved resolution, combined.Proton-transfer-reaction time-of-flight mass spectrometry has been employed in a 12 months breath gas analysis study to describe the breath composition of 19 cigarette smoking and 53 non-smoking women.

The most prevalent constituents were acetone ( ppmv), methanol ( ppbv), isoprene ( ppbv), ethanol ( ppbv), acetaldehyde (90 ppbv.